Public Health Reference laboratory (PHRL) is a reference facility for disease surveillance for the province of Khyber Pakhtunkhwa. The successful detection, characterization and tracing of disease transmission that is essential for the prevention and control of public health events requires an efficient public health laboratory (PHL) system at national and provincial levels. The PHLs generate information that is essential for guiding responses to infectious disease outbreaks by public health practitioners, epidemiologists and clinicians.